Low-Cost Methods for Molecular Characterization of Mutant Plants Tissue Desiccation, DNA Extraction and Mutation Discovery: Protocols /
This book offers low-cost and rapid molecular assays for the characterization of mutant plant germplasm. Detailed protocols are provided for the desiccation of plant tissues; the extraction of high-quality DNA for downstream applications; the extraction of single-strand-specific nucleases for single...
שמור ב:
Main Authors: | , , , , , |
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מחבר תאגידי: | |
פורמט: | אלקטרוני ספר אלקטרוני |
שפה: | אנגלית |
יצא לאור: |
Cham :
Springer International Publishing : Imprint: Springer,
2015.
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נושאים: | |
גישה מקוונת: | http://dx.doi.org/10.1007/978-3-319-16259-1 |
תגים: |
הוספת תג
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תוכן הענינים:
- Introduction
- Health and Safety Considerations
- Sample Collection and Storage
- Low-Cost DNA Extraction
- PCR Amplification for Low-Cost Mutation Discovery
- Enzymatic Mismatch Cleavage and Agarose Gel Evaluation of Samples
- Alternative Enzymology for Mismatch Cleavage for TILLING and Ecotilling: Extraction of Enzymes form Common Weedy Plants
- Example Data
- Conclusions. .