Protein Engineering Methods and Protocols /
This volume details basic and advanced protocols for both stages of protein engineering: the library design phase and the identification of improved variants by screening and selection. Chapters focus on enzyme engineering using rational and semi-rational approaches. Written in the highly successful...
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| Other Authors: | , |
| Format: | Electronic eBook |
| Language: | English |
| Published: |
New York, NY :
Springer New York : Imprint: Humana Press,
2018.
|
| Series: | Methods in Molecular Biology,
1685 |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1007/978-1-4939-7366-8 http://www.springerprotocols.com/doiresolver?genre=book&pid=10.1007/978-1-4939-7366-8 |
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| 245 | 1 | 0 | |a Protein Engineering |h [electronic resource] : |b Methods and Protocols / |c edited by Uwe T. Bornscheuer, Matthias Höhne. |
| 264 | 1 | |a New York, NY : |b Springer New York : |b Imprint: Humana Press, |c 2018. | |
| 300 | |a XI, 352 p. 82 illus., 63 illus. in color. |b online resource. | ||
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| 490 | 1 | |a Methods in Molecular Biology, |x 1064-3745 ; |v 1685 | |
| 505 | 0 | |a Protein Engineering - Past, Present, and Future -- (Semi)rational Protein Design -- Computational Analysis of Protein Tunnels and Channels -- YASARA - A Tool to Obtain Structural Guidance in Biocatalytic Investigations -- A Computational Library Design Protocol for Rapid Improvement of Protein Stability – FRESCO -- Directed Evolution of Proteins Based on Mutational Scanning -- A Brief Guide to the High-throughput Expression of Directed Evolution Libraries -- Library Growth and Protein Expression: Optimal and Reproducible Microtiter Plate Expression of Recombinant Enzymes in E. coli using MTP Shakers -- Normalized Screening of Protein Engineering Libraries by Split-GFP Crude Cell Extract Quantification -- Functional Analysis of Membrane Proteins Produced by Cell-Free Translation -- Practical Considerations Regarding the Choice of the Best High-throughput Assay -- High-throughput Screening Assays for Lipolytic Enzymes -- Continuous High-throughput Colorimetric Assays for α-transaminases -- Colorimetric High-troughput Screening Assays for the Directed Evolution of Fungal Laccases -- Directed Co-evolution of Two Cellulosic Enzymes in Escherichia coli Based on their Synergistic Reactions -- Program-guided Design of High-throughput Enzyme Screening Experiments and Automated Data Analysis/Evaluation -- Solid Phase Agar Plate Assay for Screening Amine Transaminases -- Ultrahigh-throughput Screening of Single-cell Lysates for Directed Evolution and Functional Metagenomics -- Isolation of pH-sensitive Antibody Fragments by Fluorescence-activated Cell Sorting and Yeast Surface Display -- Library Generation and Auxotrophic Selection Assays in Escherichia coli and Thermus thermophiles. . | |
| 506 | 0 | |a Open Access | |
| 520 | |a This volume details basic and advanced protocols for both stages of protein engineering: the library design phase and the identification of improved variants by screening and selection. Chapters focus on enzyme engineering using rational and semi-rational approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Protein Engineering: Methods and Protocols aims to aid scientists in the planning and performance of their experiments. . | ||
| 650 | 0 | |a Life sciences. | |
| 650 | 0 | |a Proteins. | |
| 650 | 1 | 4 | |a Life Sciences. |
| 650 | 2 | 4 | |a Protein Science. |
| 700 | 1 | |a Bornscheuer, Uwe T. |e editor. | |
| 700 | 1 | |a Höhne, Matthias. |e editor. | |
| 710 | 2 | |a SpringerLink (Online service) | |
| 773 | 0 | |t Springer eBooks | |
| 776 | 0 | 8 | |i Printed edition: |z 9781493973644 |
| 830 | 0 | |a Methods in Molecular Biology, |x 1064-3745 ; |v 1685 | |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1007/978-1-4939-7366-8 |
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