Search Results - "yeast"
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- Proteins
- Gene Expression Regulation 2
- metabolism 2
- Bioinformatics 1
- Computational Biology 1
- Computational biology 1
- DNA 1
- Databases, Protein 1
- Endoplasmic Reticulum 1
- Endoplasmic reticulum 1
- Fungal Proteins 1
- Gene Expression Regulation, Fungal 1
- Gene Regulatory Networks 1
- Genetic regulatory networks 1
- Genomics 1
- Life Sciences 1
- Life sciences 1
- Metabolism 1
- Models, Genetic 1
- Protein Folding 1
- Protein Interaction Maps 1
- Protein Science 1
- Protein Transport 1
- Protein folding 1
- Protein protein interactions 1
- Proteomics 1
- RNA Caps 1
- RNA, Messenger 1
- Saccharomyces cerevisiae 1
- Secretory Pathway 1
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Protein networks and analysis of global gene expression
Published 2007Table of Contents: “…Contents: A paradigm shift: from databases of protein sequences to protein interactions -- Technologies for measuring interactions -- Chromatin immunoprecipitation (ChIP) -- Yeast Two Hybrid (Y2H) technology -- Mass spectrometry based approaches -- Methods for querying protein networks with gene expression profiles -- Systematic validation of protein network models using gene deletions -- Cytoscape open-source software for network modeling and visualization -- Methods for network comparison across species -- Application to discover conserved protein complexes among the networks of yeast, worm, fly and Plasmodium -- Methods for querying protein networks to explain genetic (synthetic lethal) interactions -- Future directions: how network queries will enable systems biology.…”
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2
The ERAD network
Published 2012Table of Contents: “…Contents: Eukaryotic secretory pathway -- ER processes -- ERAD steps -- Substrate recognition -- Glycans as sorting signals -- Yeast ERAD process -- Mammalian ERAD process -- ERAD adapter proteins -- ERAD translocons -- ERAD E3 ligases.…”
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Electronic Video -
3
mRNA capping
Published 2007Table of Contents: “…Contents: Modification of mRNA 5' ends by addition of a m7G cap structure -- Capping for viability -- Conservation in yeast to humans -- Structure of caps and capping enzymes -- Mechanism of cap synthesis -- Protein-protein interactions that link capping to events in transcription, transport and translation -- siRNA knockdown experiments.…”
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Electronic Video -
4
Protein Engineering Methods and Protocols /
Published 2018Table of Contents: “…Protein Engineering - Past, Present, and Future -- (Semi)rational Protein Design -- Computational Analysis of Protein Tunnels and Channels -- YASARA - A Tool to Obtain Structural Guidance in Biocatalytic Investigations -- A Computational Library Design Protocol for Rapid Improvement of Protein Stability – FRESCO -- Directed Evolution of Proteins Based on Mutational Scanning -- A Brief Guide to the High-throughput Expression of Directed Evolution Libraries -- Library Growth and Protein Expression: Optimal and Reproducible Microtiter Plate Expression of Recombinant Enzymes in E. coli using MTP Shakers -- Normalized Screening of Protein Engineering Libraries by Split-GFP Crude Cell Extract Quantification -- Functional Analysis of Membrane Proteins Produced by Cell-Free Translation -- Practical Considerations Regarding the Choice of the Best High-throughput Assay -- High-throughput Screening Assays for Lipolytic Enzymes -- Continuous High-throughput Colorimetric Assays for α-transaminases -- Colorimetric High-troughput Screening Assays for the Directed Evolution of Fungal Laccases -- Directed Co-evolution of Two Cellulosic Enzymes in Escherichia coli Based on their Synergistic Reactions -- Program-guided Design of High-throughput Enzyme Screening Experiments and Automated Data Analysis/Evaluation -- Solid Phase Agar Plate Assay for Screening Amine Transaminases -- Ultrahigh-throughput Screening of Single-cell Lysates for Directed Evolution and Functional Metagenomics -- Isolation of pH-sensitive Antibody Fragments by Fluorescence-activated Cell Sorting and Yeast Surface Display -- Library Generation and Auxotrophic Selection Assays in Escherichia coli and Thermus thermophiles. .…”
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