Search Results - "yeast"

START control in yeast

Table of Contents: “…Contents: Control of cell proliferation -- The budding yeast: Saccharomyces cerevisiae -- Why yeast? -- The budding yeast life cycle -- Placement of START in the cell cycle -- Observing START -- The G1 Checkpoint -- Genetic characterization of START -- Interdependency of Cdk1 with other regulators of START -- Cyclin-Dependent Protein Kinase (CDK) -- The cyclin/CDK cycle -- G1 cyclin/CDK is essential for START -- The role of CDK in START -- Cell cycle dependent expression of Far1 and cyclins -- Periodic accumulation of yeast cell cycle regulators -- The molecular correlate of START -- G1-specific transcription -- G1-specific gene expression and human cancer -- The molecular determinant of START -- Physiological regulators of START -- Mating generates diploid cells from two haploid cells -- Cells of opposite mating types can conjugate -- Stimulation of the mating pheromone cascade elicits three primary responses -- Mating pheromone signaling pathway.…”
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Chromosome bi-orientation in yeast

Table of Contents: “…-- Failure to bi-orient a chromosome will lead to mis-segregation/aneuploidy -- Modes of sister chromatid attachment to spindle microtubules -- The budding yeast (Saccharomyces cerevisiae) as a model for studying chromosome bi-orientation -- Monitoring sister centromeres on individual yeast chromosomes -- Kinetochore capture -- Achieving chromosome bi-orientation: mechanisms -- Geometry-dependent mechanism or a correction mechanism? …”
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Spindle movement and checkpoint control during mitosis in yeast

Table of Contents: “…Contents: Cell cycle checkpoints for partitioning the genome -- Budding yeast as a model system for the study of genome distribution -- How nuclei are segregated in yeast -- Evidence for a spindle position checkpoint -- Model for checkpoint based on Tem1 -- Movie assay for checkpoint -- Search for upstream inputs -- Screen for checkpoint mutants -- Septins dual role in checkpoint -- Inputs to Lte1 -- Bud6 and microtubule capture -- Potential pathway for Bud6 -- Bud6 and microtubule-binding proteins -- Overexpression analysis -- Inhibition of Lte1 in the cold.…”
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Initiation of DNA replication

Table of Contents: “…Contents: Cell cycle control of DNA replication -- Yeast as a model eukaryote -- Control of S-phase entry in yeast -- Characterization of origins of DNA replication -- Modular structure of S. cerevisiae origins -- The budding yeast origin recognition complex (ORC) -- Structure of the ORC-Cdc6 complex -- Evolutionary comparison of ORC AAA+ subunits -- Proteins required for formation of the pre-Replication Complex (pre-RC) -- Cell cycle dependent association of yeast initiation proteins with chromatin -- ATP-dependent assembly of a pre-RC -- Cell cycle regulation of the pre-RC -- The chromosome cycle and establishment of pre-RCs -- Dynamic assembly and stability of ORC in human cells -- Commitment to S-phase and initiation -- Cdc45p is associated with chromatin in a cell cycle-dependent manner -- Temporal regulation of DNA replication -- Dynamics of pre-RC proteins in human cells differ from pre-RC proteins in yeast.…”
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Replication licensing

Table of Contents: “…Contents: Problems in ensuring complete genome replication -- Experiments revealing the existence of a replication licence -- The licensing reaction -- ORC, Cdc6, Cdt1 and Mcm2-7 -- Cell cycle regulation of licensing -- Pre-RC substrates in yeast -- Regulation of licensing in animal cells by Cdt1 degradation and geminin inhibition -- Is there a licensing checkpoint? …”
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The spindle checkpoint

Table of Contents: “…-- Unattached kinetochores -- Lack of tension -- Checkpoint components -- Budding yeast genetic screens -- Microcolony assay -- Localisation of Mad proteins -- Checkpoint proteins are all recruited kinetochores -- Role of kinetochores -- Global signaling -- Identification of Cdc20 as the checkpoint effector -- CdC20-APC -- FRAP -- Mad1/Mad2 complex as a template -- The anaphase promoting complex (APC) -- How is anaphase onset inhibited -- Mad2 and Mad3 bind mitotic APC -- Separase regulation by securin -- Error correction -- Chromosomal passenger proteins -- How to sense a lack of tension? …”
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Geometric regulation of kinetochore orientation

Table of Contents: “…Contents: The cell cycle -- Aurora B-dependent destabilization -- The attachment is stabilized under tension -- Tension-dependent reorientation -- Kinetochore geometry -- Organization of centromeres -- Budding yeast -- Two models to account for the mono-polar attachment at meiosis I -- Rec8 vs. …”
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