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Assays for cytokines and cytokine assay standardization

Table of Contents: “…Contents: The importance of measuring cytokines -- The types of cytokine assays available -- Examples of most widely used cytokine assays -- Principles of ELISA and ELISPOT -- How to select the right cytokine assay -- Multiplex analyses and cytokine profiling -- Principles of QA/QC for cytokine assays -- Data analysis and pitfalls.…”
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Assays for cytokines and cytokine assay performance part 2 of 2 /

Table of Contents: “…Contents: Types of cytokine assays available -- Examples of most widely used cytokine assays -- Principles of ELISA and ELISPOT -- How to select the right cytokine assay -- Multiplex analyses and cytokine profiling -- Principles of QA/QC for cytokine assays -- Data analysis and pitfalls.…”
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Assays for cytokines and cytokine assay performance part 1 of 2 /

Table of Contents: “…Contents: The importance of measuring cytokines -- Features of cytokines relevant to assays -- Types of cytokine assays -- Examples of most widely used cytokine assays -- Principles of ELISA -Selecting the correct cytokine assay -- Multiplex analyses and cytokine profiles.…”
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Hematopoietic stem cells the power of clonal assays /

Table of Contents: “…Contents: The haematopoietic process -- Spleen colony assay -- Colony forming assay -- Competition repopulation assay -- Haematopoietic stem cell subtypes and leukemia -- Index sorting experiments and the identification of a molecular overlap -- Assessing somatic mutations within HSC colonies -- Recapture experiments -- Inferring HSC population size.…”
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Oxidatively generated damage to DNA mechanistic aspects and formation in cells /

Table of Contents: “…Contents: Oxidative stress and cellular oxidants -- Nucleobase degradation pathways induced by hydroxyl radical, singlet oxygen and one-electron oxidants -- Oxidatively generated damage to cellular DNA: single and tandem lesions -- Epigenetic marks: enzymatic oxidation of 5-methycytosine -- Measurement of oxidized bases: chromatographic and enzymatic assays -- Base oxidation products formed in cellular DNA upon exposure to ionizing radiations and UVA component of solar light.…”
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G-Protein coupled receptors in drug discovery

Subjects: “…High-Throughput Screening Assays. 104620…”
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Virtual screening

Subjects: “…High-Throughput Screening Assays. 85836…”
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The classical pathway of complement

Table of Contents: “…Contents: Three activation pathways of complement -- Initiation of the classical pathway -- Dose-dependent effects of terminal complement complexes -- Hemolytic assays and ELISA for quantification of complement activity -- Clearance of self-debris and of immune complexes -- Complement deficiency and autoimmunity -- Disturbed apoptotic cell processing -- Systemic Lupus Erythematosus -- Fluid phase and membrane-bound complement regulators.…”
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In vivo antibody discovery and hybridoma technology

Subjects: “…Enzyme-Linked Immunosorbent Assay. 83281…”
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A chemical approach to controlling cell fate

Subjects: “…High-Throughput Screening Assays. 104339…”
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Ligand-based drug design

Subjects: “…High-Throughput Screening Assays. 96999…”
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Accelerating drug discovery with machine learning and AI

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Novel approaches to diagnosis of viral infections

Table of Contents: “…Contents: Introduction to microbial discovery, surveillance and diagnosis -- Koch's Postulates and proof of causation -- Mechanisms of microbial pathogenesis -- Gene:environment interactions -- Molecular and serological assays for differential diagnosis of infectious diseases -- High throughput sequencing -- Future perspectives.…”
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Genome scale analysis of cellular processes using RNAi

Table of Contents: “…Contents: Functional analysis in Drosophila -- RNAi -- Genetic screens by RNAi -- RNAi libraries -- Cell-based assays -- Screen analysis -- Systematic analysis of high-throughput data -- Normalization and hit identification -- Challenges in quantitative image analysis -- Issues and insights from screen results -- Applications for RNAi screens -- Assay for cell fitness -- Assay for cell morphology -- Shared defects and shared pathways -- Outlook for RNAi screens.…”
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Cell signalling pathways and RNAi

Table of Contents: “…Contents: Use of functional cell-based reporter system (Cell Sensor(tm) technology) with RNAi -- From target knockdown to compound screening and verification in biochemical assays -- Analysis of small-molecule protein kinase inhibitors -- Epidermal growth factor receptor (EGFR) signalling pathway -- StealthTM RNAi, second-generation RNAi compounds -- Integration of a cell-based assay system, RNAi and biochemical assay technologies -- Kinase discovery platform to identify biologically relevant kinase targets -- Characterization of kinase inhibitors in systems -- Possible synergistic effects of combining RNAi and kinase inhibitors.…”
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Principles of virology II

Table of Contents: “…Contents: Virus cultivation -- Virus isolation -- Animal hosts -- Cell culture -- Recognition of virus infection in culture -- CPE: morphological changes -- Hemadsorption -- Biological assay of viruses -- Plaque assay -- Focus assay -- Endpoint method -- Physical assay of viruses -- Hemagglutination -- Direct particle count -- Virus genetics -- Genetic principles -- Mutation -- Engineered host range -- Temperature sensitive mutants -- Antiviral drugs -- Uses of drug resistance/dependence -- Recombination and reassortment -- Reverse genetics.…”
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Genome-wide analyses of protein-DNA interactions

Table of Contents: “…Contents: Identifying genomic targets of DNA-binding factors -- Experimental methods -- Chromatin immunoprecipitation (ChIP) assay -- Probing microarrays -- ChIP-on-chip assays for comprehensive analysis -- Data interpretation and follow-up studies.…”
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Visualization of transcription factor interactions in living cells

Table of Contents: “…Contents: Transcription regulatory proteins -- Combinatorial control of gene expression -- Visualization of transcription factor interactions and modifications in living cells -- New methods development -- Bimolecular fluorescence complementation (BiFC) assays -- Ubiquitin mediated fluorescence complementation (UbFC) assays.…”
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Series (Eukaryotic gene regulation)
Series (Epigenetics, chromatin, transcription and cancer)

Laboratory methods for the in vitro study of drug metabolism

Table of Contents: “…Contents: Major classes of drug-metabolizing enzymes -- Co-factor requirement for in vitro incubations -- Practical aspects of conducting in vitro assays -- In vitro assays conducted during drug discovery and development -- Clearance estimation -- Kinetic parameter determination -- Enzyme inhibition -- Enzyme induction -- Enzyme reaction phenotyping.…”
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