Search Results - "yeast"

The yeast plasmid a highly persistent selfish DNA element /

Table of Contents: “…Contents: The yeast plasmid as a highly optimized design for a multi-copy selfish DNA element -- The need for an active partitioning system for the segregation of a high copy plasmid -- Coupling between plasmid segregation and chromosome segregation -- Poaching of host factors required for chromosome segregation by the plasmid partitioning system -- Cohesin mediated equal segregation of duplicated plasmid clusters.…”
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Yeast and fungal prions a help or a hindrance? /

“…Prions and amyloids : self-propagating protein structures in mammals, yeast and fungi.…”
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START control in yeast

Table of Contents: “…Contents: Control of cell proliferation -- The budding yeast: Saccharomyces cerevisiae -- Why yeast? -- The budding yeast life cycle -- Placement of START in the cell cycle -- Observing START -- The G1 Checkpoint -- Genetic characterization of START -- Interdependency of Cdk1 with other regulators of START -- Cyclin-Dependent Protein Kinase (CDK) -- The cyclin/CDK cycle -- G1 cyclin/CDK is essential for START -- The role of CDK in START -- Cell cycle dependent expression of Far1 and cyclins -- Periodic accumulation of yeast cell cycle regulators -- The molecular correlate of START -- G1-specific transcription -- G1-specific gene expression and human cancer -- The molecular determinant of START -- Physiological regulators of START -- Mating generates diploid cells from two haploid cells -- Cells of opposite mating types can conjugate -- Stimulation of the mating pheromone cascade elicits three primary responses -- Mating pheromone signaling pathway.…”
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Yeast studies on ageing

Table of Contents: “…Contents: History of studies on yeast ageing -- Senescence factor, molecular memory, and age locus -- Mitochondria and ageing -- Age asymmetry and cell quality control -- Epigenetic gene regulation and ageing -- Genome instability as a cause of ageing -- Replicative and chronological lifespans -- Nutrient effects on lifespan -- Ageing in the wild.…”
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Gene silencing in budding yeast

Table of Contents: “…Contents: Gene silencing: an epigenetic phenomenon -- Chromatin remodeling: heterochromatin and euchromatin -- Silent chromatin in budding yeast: mating-type loci -- Silent information regulation: telomeric genes and Sir proteins.…”
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Mechanisms of yeast prion propagation

Table of Contents: “…Contents: Prions as infectious amyloids -- Yeast [PSI+] prion and Sup35p -- Molecular chaperone Hsp104 -- Guanidine hydrochloride as a prion propagation inhibitor -- Propagons -- Estimating propagon numbers using a stochastic model -- Generation of new propagons -- Propagation-defective mutants (PNM) -- Role of primary sequence information -- Yeast prion propagation: the unanswered questions.…”
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Yeast prions and protein chaperones a help or a hindrance? /

Table of Contents: “…Contents: Role of protein chaperones in protein folding -- Yeast prions as amyloid forms of cellular proteins -- Requirement of chaperone machinery for prion propagation -- Chaperone mutants adversely affect prions and provide insight into chaperone/amyloid interactions.…”
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Chromosome bi-orientation in yeast

Table of Contents: “…-- Failure to bi-orient a chromosome will lead to mis-segregation/aneuploidy -- Modes of sister chromatid attachment to spindle microtubules -- The budding yeast (Saccharomyces cerevisiae) as a model for studying chromosome bi-orientation -- Monitoring sister centromeres on individual yeast chromosomes -- Kinetochore capture -- Achieving chromosome bi-orientation: mechanisms -- Geometry-dependent mechanism or a correction mechanism? …”
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RNAi and heterochromatin in plants and fission yeast

Table of Contents: “…Contents: Heterochromatin is composed of transposable elements (TEs) and related repeats -- heterochromatic gene silencing and TE-mediated silencing are related and may be important in large genomes -- tiling microarrays can be used to examine heterochromatic transcripts as well as DNA and histone modification -- small interfering RNA (siRNA) corresponds to transposons and repeats -- in plants TE siRNA depend on DNA methyltransferase MET1 and the SWI/SNF ATPase DDM1 which silence TEs via DNA and histone H3 lysine-9 (H3K9) methylation -- in fission yeast and plants centromeric repeats are transcribed on one strand but rapidly turned over by RNA interference (RNAi) -- RNAi of centromeric transcripts is required for transcriptional silencing of reporter genes -- RNA polymerase II, the Argonaute (RITS) and RNA dependent RNA polymerase (RDRC) complexes are associated with heterochromatin and required for silencing -- H3K9me2 depends on RNAi and on the Rik1-Clr4 complex -- Clr4 is the histone H3K9 dimethyltransferase -- Rik1 resembles both DNA and RNA binding proteins and is required for RNAi along with Clr4 -- LTR retrotransposon silencing depends on histone deacetylation and silences pericentromeric repeats in Arabidopsis in addition to RNAi.…”
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Roles of glycans in yeast cell walls

Table of Contents: “…Contents: The overall architecture of yeast cell walls and the roles of glycans -- Structure and biosynthesis of major cell wall components -- A scheme for cell wall assembly and crosslinking -- Cell wall dynamics in growth, budding, stress response, cell adhesion, and mating -- Cell wall structure in other fungi and a relationship among eukaryotic extracellular matrices.…”
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Propagation and variability of the yeast [PSI+] prion

Table of Contents: “…Contents: Prion -- Yeast prions as non-chromosomal genetic determinants -- Modular structure of the Sup35 protein responsible for the yeast [PSI+] prion -- Role of the Hsp104 chaperone in [PSI+] prion propagation -- Two-level structure of the [PSI+] prion aggregates and methods for studying the size of prion polymers -- Non-prion amyloids in yeast -- Variability of the yeast [PSI+] prion.…”
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Structure of cell walls in yeast and other fungi

Table of Contents: “…Contents: Fungal cell walls -- Glycans -- S. cerevisiae -- C. albicans -- Mannose -- Chitin -- Glycan biosynthesis -- Mannoproteins -- GPI-crosslinked proteins -- N. crassa -- Yeast cell wall dynamics -- Cell wall conservation and evolution.…”
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Mapping the molecular chaperone interaction network in yeast

Table of Contents: “…Contents: Large scale proteomic screening in yeast -- The TAP-tag pulldown approach -- Understanding the principles governing protein homeostasis -- Mapping the network of the Hsp90 chaperone using physical and genetic interaction screens -- Unraveling the role of Hsp90 in pre-rRNA processing.…”
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Organellar calcium pumps of the golgi and ER from yeast to human /

Table of Contents: “…Contents: Early and current views on Calcium signaling -- Phylogenetic tree of Calcium-ATPases -- Identification of a new family of Golgi/secretory pathway Calcium-ATPases (SPCA) -- Why have a Calcium pump in the Golgi? -- Yeast as a model to understand Golgi Calcium pumps -- Biochemical characterization of Calcium and Mn2+ transport -- Phenotype screening in yeast to identify residues important for ion transport and selectivity in Pmr1 -- Structural and homology models of Calcium-ATPases -- Golgi pumps in mammals -- From structure to physiology -- Haploinsufficiency of ATP2C1 leads to Hailey Hailey disease -- Insights from yeast -- Distribution and localization of SPCA1 and SPCA2.…”
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Spindle movement and checkpoint control during mitosis in yeast

Table of Contents: “…Contents: Cell cycle checkpoints for partitioning the genome -- Budding yeast as a model system for the study of genome distribution -- How nuclei are segregated in yeast -- Evidence for a spindle position checkpoint -- Model for checkpoint based on Tem1 -- Movie assay for checkpoint -- Search for upstream inputs -- Screen for checkpoint mutants -- Septins dual role in checkpoint -- Inputs to Lte1 -- Bud6 and microtubule capture -- Potential pathway for Bud6 -- Bud6 and microtubule-binding proteins -- Overexpression analysis -- Inhibition of Lte1 in the cold.…”
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MAPK signalling regulation and cancer lessons from fission yeast /

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Chaperones and prions

Table of Contents: “…Contents: Prion diseases -- Prions as self-propagating amyloids -- Yeast prions as protein-based heritable elements -- Prion detection -- Crucial role of the chaperone protein Hsp104 in the propagation of yeast prions -- Chaperone-mediated fragmentation turns amyloids into infectious or heritable prions -- Effects of the Hsp70 and Hsp40 proteins on yeast prions -- Other modulators of prion formation and propagation: cytoskeleton and ubiquitin system.…”
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Impact of systems biology on metabolic engineering

Table of Contents: “…Contents: The biorefinery concept -- The process of metabolic engineering (ME) -- Approaches in systems biology (SB) -- Genome scale metabolic models (GEMs) -- Using SB to design succinic acid production in yeast -- Using SB to compare metabolism of yeast strains -- Using SB to compare penicillin producing in yeast strains -- Inverse metabolic engineering -- Using inverse ME to improve galactose utilization in yeast -- Mechanisms of evolutionary trade-offs.…”
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A novel cancer therapy to stimulate oncogenic ERK signalling

Table of Contents: “…Contents: MAPK signalling and its relevance to cancer therapy -- The discovery of ACA-28 -- The use of yeast within drug discovery -- Effect of ACA-28 on the apoptosis of cancer cells -- Implications of ACA-28 in cancer therapeutics.…”
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Mechanism of prion generation in vitro

“…Prions and amyloids : self-propagating protein structures in mammals, yeast and fungi.…”
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