Search Results - "living cells"

Monitoring Ca2+ concentration in living cells

“…Monitoring calcium concentration in living cells…”
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Visualization of transcription factor interactions in living cells

Table of Contents: “…Contents: Transcription regulatory proteins -- Combinatorial control of gene expression -- Visualization of transcription factor interactions and modifications in living cells -- New methods development -- Bimolecular fluorescence complementation (BiFC) assays -- Ubiquitin mediated fluorescence complementation (UbFC) assays.…”
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Series (Eukaryotic gene regulation)
Series (Epigenetics, chromatin, transcription and cancer)

Cellular signaling on host-malaria parasite interactions

Table of Contents: “…Contents: Plasmodium signaling -- Control of the cell cycle mechanisms -- Clinical and diagnostic applications in malarial research -- Live cell microscope work -- Molecular techniques.…”
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Mitochondrial heterogeneity in cells

Table of Contents: “…Contents: Levels of mitochondrial heterogeneity -- Subcellular heterogeneity -- Membrane potential -- Permeability transition -- Calcium uptake -- Morphology: network or discrete? -- Live cell mitochondrial 3D reconstruction -- Fluorescence Recovery After Photobleaching (FRAP) -- Electrical continuity -- Atypical networks.…”
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Nuclear organization and gene expression

Table of Contents: “…Contents: Current understanding of the organization of the mammalian cell nucleus and how this organization impacts on gene expression -- in-vivo studies of gene expression as a result of the concerted processes of transcription, mRNA processing, messenger ribonucleoprotein particle (mRNP) export and translation -- studies of spatial and temporal aspects of critical cellular processes within the context of the living cell -- development of a cell system that allows the visualization of a transcription site and its mRNA and protein products directly in living cells -- evaluation of spatial and temporal changes in chromatin structure, mRNA synthesis and factor association/disassociation during the transition from a transcriptionally inactive to an active state -- understanding of the in-vivo dynamics of gene expression will provide the basis from which to elucidate spatial and/or temporal alterations that occur in cells associated with various disease states.…”
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Series (Epigenetics)
Series (From DNA to proteins)

Network motifs basic building blocks of biological networks /

Table of Contents: “…-- Database of direct transcription interactions in E. coli -- Algorithm that finds n-node network motifs -- Feedforward loop (FFL) -- Dynamics of the feed-forward loop system -- The feed-forward loop is a filter for transient signals allowing fast shutdown -- GFP reporter plasmid system for promoter activity -- Response to brief pulses of X is filtered by FFL -- Sign-sensitive filtering by arabinose feed-forward loop -- Single input module (SIM) -- Dense overlapping regulons (DOR) -- Incoherent FFL is a pulse generator -- Network motifs in human organ development -- Network motifs in growth factor signalling -- High-accuracy promoter activity measurements in living cells.…”
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Deciphering neurodegeneration models and methods /

Table of Contents: “…Contents: Deciphering Neurodegeneration: Models and Methods -- The challenge of neurodegeneration -- Huge diversity in cell types -- Nature versus nurture -- Reductionism versus systems biology -- Cellular models -- Yeast -- Primary cells -- Cell lines -- Stem cells -- Model organisms -- Caenorhabditis elegans -- Drosophila melanogaster -- Zebrafish -- Danio rerio -- Rodents -- Molecular biology approaches -- Knock out and knock in -- Genomic editing -- RNAi -- Overexpression -- Gene expression -- rtPCR -- Microarrays -- RNAseq -- Protein analysis -- Immunoblot -- ELISA -- Mass spec and proteomics -- Microscopy -- Immunocytochemistry -- Live cell imaging -- Electrophysiology.…”
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