Utilizing microarrays to study posttranscriptional regulatory mechanisms of plants

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Bibliographic Details
Other Authors: Gregory, Brian D. (Speaker)
Format: Electronic Video
Language:English
Published: London : Henry Stewart Talks, 2009.
Series:Henry Stewart talks. Biomedical & life sciences collection. Microarrays : their design and use.
Subjects:
Online Access:https://hstalks.com/bs/1205/
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Table of Contents:
  • Contents: mRNA structure
  • The cap-binding complex is required for proper mRNP assembly
  • Phenotypes of abh1-1 mutant plants
  • Altered phyllotaxy in abh1-1 mutant plants
  • RNA silencing in plants
  • Arabidopsis whole genome tiling array
  • High-throughput sequencing of the smRNA-component of the Arabidopsis transcriptome
  • ABH1 is required for proper mature miRNA levels
  • 5'-RACE experiments
  • Loss of ABH1 enhances the developmental defects of a hypomorphic AGO1 allele
  • RNA degradation
  • Exosome function in Arabidopsis
  • Tiling arrays suggest the exosome regulates specific fragments of primary miRNA transcripts
  • Many of the new transcription units only seen upon exosome-depletion are associated with smRNAs and repetitive elements
  • A genome-wide atlas of exosome substrates in Arabidopsis.