Plasmid rolling-circle (RC) replication
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| Format: | Electronic Video |
| Language: | English |
| Published: |
London :
Henry Stewart Talks,
2008.
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| Series: | Henry Stewart talks. Biomedical & life sciences collection. Biology and significance of plasmids : a discussion of extrachromosomal elements in microorganisms.
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| Subjects: | |
| Online Access: | https://hstalks.com/bs/703/ Series |
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Table of Contents:
- Contents: Rolling circle replicating (RCR) plasmids
- Functional organization of rolling-circle replicating plasmids
- Model for plasmid rolling-circle replication
- Initiation and termination of replication in plasmids carrying two pT181 dsos
- DNA sequence and structure of the pT181 origin
- Initiator proteins of RCR plasmids
- Comparison of the replication origins of the plasmids of pT181 family
- Rep-DNA specificity
- Role of individual monomers of the Rep protein
- Purification of RepC heterodimers
- PcrA helicase
- Role of PcrA helicase in plasmid RC replication
- Conversion of ss to ds DNA
- Single strand origins of replication
- Lagging-strand replication.
Replication of linear plasmids in bacteria