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Low-Cost Methods for Molecular Characterization of Mutant Plants Tissue Desiccation, DNA Extraction and Mutation Discovery: Protocols /

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This book offers low-cost and rapid molecular assays for the characterization of mutant plant germplasm. Detailed protocols are provided for the desiccation of plant tissues; the extraction of high-quality DNA for downstream applications; the extraction of single-strand-specific nucleases for single...

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Autori principali: Till, Bradley J. (Autore), Jankowicz-Cieslak, Joanna (Autore), Huynh, Owen A. (Autore), Beshir, Mayada M. (Autore), Laport, Robert G. (Autore), Hofinger, Bernhard J. (Autore)
Ente Autore: SpringerLink (Online service)
Natura: Elettronico eBook
Lingua:inglese
Pubblicazione: Cham : Springer International Publishing : Imprint: Springer, 2015.
Soggetti:
Life sciences.
Nucleic acids.
Plant breeding.
Biology > Technique.
Life Sciences.
Plant Breeding/Biotechnology.
Biological Techniques.
Nucleic Acid Chemistry.
Accesso online:http://dx.doi.org/10.1007/978-3-319-16259-1
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Sommario:
  • Introduction
  • Health and Safety Considerations
  • Sample Collection and Storage
  • Low-Cost DNA Extraction
  • PCR Amplification for Low-Cost Mutation Discovery
  • Enzymatic Mismatch Cleavage and Agarose Gel Evaluation of Samples
  • Alternative Enzymology for Mismatch Cleavage for TILLING and Ecotilling: Extraction of Enzymes form Common Weedy Plants
  • Example Data
  • Conclusions.  .

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