Low-Cost Methods for Molecular Characterization of Mutant Plants Tissue Desiccation, DNA Extraction and Mutation Discovery: Protocols /
This book offers low-cost and rapid molecular assays for the characterization of mutant plant germplasm. Detailed protocols are provided for the desiccation of plant tissues; the extraction of high-quality DNA for downstream applications; the extraction of single-strand-specific nucleases for single...
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| Auteurs principaux: | , , , , , |
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| Collectivité auteur: | |
| Format: | Électronique eBook |
| Langue: | anglais |
| Publié: |
Cham :
Springer International Publishing : Imprint: Springer,
2015.
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| Sujets: | |
| Accès en ligne: | http://dx.doi.org/10.1007/978-3-319-16259-1 |
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Table des matières:
- Introduction
- Health and Safety Considerations
- Sample Collection and Storage
- Low-Cost DNA Extraction
- PCR Amplification for Low-Cost Mutation Discovery
- Enzymatic Mismatch Cleavage and Agarose Gel Evaluation of Samples
- Alternative Enzymology for Mismatch Cleavage for TILLING and Ecotilling: Extraction of Enzymes form Common Weedy Plants
- Example Data
- Conclusions. .