Protein Engineering Methods and Protocols /
This volume details basic and advanced protocols for both stages of protein engineering: the library design phase and the identification of improved variants by screening and selection. Chapters focus on enzyme engineering using rational and semi-rational approaches. Written in the highly successful...
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| Other Authors: | , |
| Format: | Electronic eBook |
| Language: | English |
| Published: |
New York, NY :
Springer New York : Imprint: Humana Press,
2018.
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| Series: | Methods in Molecular Biology,
1685 |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1007/978-1-4939-7366-8 http://www.springerprotocols.com/doiresolver?genre=book&pid=10.1007/978-1-4939-7366-8 |
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Table of Contents:
- Protein Engineering - Past, Present, and Future
- (Semi)rational Protein Design
- Computational Analysis of Protein Tunnels and Channels
- YASARA - A Tool to Obtain Structural Guidance in Biocatalytic Investigations
- A Computational Library Design Protocol for Rapid Improvement of Protein Stability – FRESCO
- Directed Evolution of Proteins Based on Mutational Scanning
- A Brief Guide to the High-throughput Expression of Directed Evolution Libraries
- Library Growth and Protein Expression: Optimal and Reproducible Microtiter Plate Expression of Recombinant Enzymes in E. coli using MTP Shakers
- Normalized Screening of Protein Engineering Libraries by Split-GFP Crude Cell Extract Quantification
- Functional Analysis of Membrane Proteins Produced by Cell-Free Translation
- Practical Considerations Regarding the Choice of the Best High-throughput Assay
- High-throughput Screening Assays for Lipolytic Enzymes
- Continuous High-throughput Colorimetric Assays for α-transaminases
- Colorimetric High-troughput Screening Assays for the Directed Evolution of Fungal Laccases
- Directed Co-evolution of Two Cellulosic Enzymes in Escherichia coli Based on their Synergistic Reactions
- Program-guided Design of High-throughput Enzyme Screening Experiments and Automated Data Analysis/Evaluation
- Solid Phase Agar Plate Assay for Screening Amine Transaminases
- Ultrahigh-throughput Screening of Single-cell Lysates for Directed Evolution and Functional Metagenomics
- Isolation of pH-sensitive Antibody Fragments by Fluorescence-activated Cell Sorting and Yeast Surface Display
- Library Generation and Auxotrophic Selection Assays in Escherichia coli and Thermus thermophiles. .