RNAi and heterochromatin in plants and fission yeast
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| Other Authors: | |
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| Format: | Electronic Video |
| Language: | English |
| Published: |
London :
Henry Stewart Talks,
2007.
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| Series: | Henry Stewart talks. Biomedical & life sciences collection. Epigenetics : concepts, theories, paradigms and mechanisms.
Henry Stewart talks. Biomedical & life sciences collection. RNA interference : principles and applications. |
| Subjects: | |
| Online Access: | https://hstalks.com/bs/112/ Series (Epigenetics) Series (RNA interference) |
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Table of Contents:
- Contents: Heterochromatin is composed of transposable elements (TEs) and related repeats
- heterochromatic gene silencing and TE-mediated silencing are related and may be important in large genomes
- tiling microarrays can be used to examine heterochromatic transcripts as well as DNA and histone modification
- small interfering RNA (siRNA) corresponds to transposons and repeats
- in plants TE siRNA depend on DNA methyltransferase MET1 and the SWI/SNF ATPase DDM1 which silence TEs via DNA and histone H3 lysine-9 (H3K9) methylation
- in fission yeast and plants centromeric repeats are transcribed on one strand but rapidly turned over by RNA interference (RNAi)
- RNAi of centromeric transcripts is required for transcriptional silencing of reporter genes
- RNA polymerase II, the Argonaute (RITS) and RNA dependent RNA polymerase (RDRC) complexes are associated with heterochromatin and required for silencing
- H3K9me2 depends on RNAi and on the Rik1-Clr4 complex
- Clr4 is the histone H3K9 dimethyltransferase
- Rik1 resembles both DNA and RNA binding proteins and is required for RNAi along with Clr4
- LTR retrotransposon silencing depends on histone deacetylation and silences pericentromeric repeats in Arabidopsis in addition to RNAi.
RNAi and heterochromatin in plants and fission yeast