11β-hydroxysteroid dehydrogenase-1 deficiency alters the gut microbiome response to Western diet
The enzyme 11β-hydroxysteroid dehydrogenase (11β-HSD) interconverts active glucocorticoids and their intrinsically inert 11-keto forms. The type 1 isozyme, 11β-HSD1, predominantly reactivates glucocorticoids in vivo and can also metabolise bile acids. 11β-HSD1-deficient mice show altered inflammator...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
The Journal of Endocrinology
2019
|
| Subjects: | |
| Online Access: | https://repository.daystar.ac.ke/handle/123456789/2965 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Add Tag | _version_ | 1792071293918511104 |
|---|---|
| author | Jethro, S Johnson Opiyo, Monica N Thomson, Marian Karim, Gharbi Andreas, Heger Chapman, Karen E |
| author_facet | Jethro, S Johnson Opiyo, Monica N Thomson, Marian Karim, Gharbi Andreas, Heger Chapman, Karen E |
| author_sort | Jethro, S Johnson |
| collection | DSpace |
| description | The enzyme 11β-hydroxysteroid dehydrogenase (11β-HSD) interconverts active glucocorticoids and their intrinsically inert 11-keto forms. The type 1 isozyme, 11β-HSD1, predominantly reactivates glucocorticoids in vivo and can also metabolise bile acids. 11β-HSD1-deficient mice show altered inflammatory responses and are protected against the adverse metabolic effects of a high-fat diet. However, the impact of 11β-HSD1 on the composition of the gut microbiome has not previously been investigated. We used high-throughput 16S rDNA amplicon sequencing to characterise the gut microbiome of 11β-HSD1-deficient and C57Bl/6 control mice, fed either a standard chow diet or a cholesterol- and fat-enriched ‘Western’ diet. 11β-HSD1 deficiency significantly altered the composition of the gut microbiome, and did so in a diet-specific manner. On a Western diet, 11β-HSD1 deficiency increased the relative abundance of the family Bacteroidaceae, and on a chow diet, it altered relative abundance of the family Prevotellaceae. Our results demonstrate that (i) genetic effects on host–microbiome interactions can depend upon diet and (ii) that alterations in the composition of the gut microbiome may contribute to the aspects of the metabolic and/or inflammatory phenotype observed with 11β-HSD1 deficiency. |
| format | Article |
| id | oai:repository.daystar.ac.ke:123456789-2965 |
| institution | Daystar University |
| language | English |
| publishDate | 2019 |
| publisher | The Journal of Endocrinology |
| record_format | dspace |
| spelling | oai:repository.daystar.ac.ke:123456789-29652024-02-21T16:24:22Z 11β-hydroxysteroid dehydrogenase-1 deficiency alters the gut microbiome response to Western diet Jethro, S Johnson Opiyo, Monica N Thomson, Marian Karim, Gharbi Andreas, Heger Chapman, Karen E Glucocorticoids 11β-HSD Diet Gut microbiome The enzyme 11β-hydroxysteroid dehydrogenase (11β-HSD) interconverts active glucocorticoids and their intrinsically inert 11-keto forms. The type 1 isozyme, 11β-HSD1, predominantly reactivates glucocorticoids in vivo and can also metabolise bile acids. 11β-HSD1-deficient mice show altered inflammatory responses and are protected against the adverse metabolic effects of a high-fat diet. However, the impact of 11β-HSD1 on the composition of the gut microbiome has not previously been investigated. We used high-throughput 16S rDNA amplicon sequencing to characterise the gut microbiome of 11β-HSD1-deficient and C57Bl/6 control mice, fed either a standard chow diet or a cholesterol- and fat-enriched ‘Western’ diet. 11β-HSD1 deficiency significantly altered the composition of the gut microbiome, and did so in a diet-specific manner. On a Western diet, 11β-HSD1 deficiency increased the relative abundance of the family Bacteroidaceae, and on a chow diet, it altered relative abundance of the family Prevotellaceae. Our results demonstrate that (i) genetic effects on host–microbiome interactions can depend upon diet and (ii) that alterations in the composition of the gut microbiome may contribute to the aspects of the metabolic and/or inflammatory phenotype observed with 11β-HSD1 deficiency. 2019-09-04T09:44:15Z 2019-09-04T09:44:15Z 2016-12 Article 11β-hydroxysteroid dehydrogenase-1 deficiency alters the gut microbiome response to Western diet by Opiyo,N(et...al),The Journal of Endocrinology,2016. doi: 10.1530/JOE-16-0578 https://repository.daystar.ac.ke/handle/123456789/2965 en application/pdf The Journal of Endocrinology |
| spellingShingle | Glucocorticoids 11β-HSD Diet Gut microbiome Jethro, S Johnson Opiyo, Monica N Thomson, Marian Karim, Gharbi Andreas, Heger Chapman, Karen E 11β-hydroxysteroid dehydrogenase-1 deficiency alters the gut microbiome response to Western diet |
| title | 11β-hydroxysteroid dehydrogenase-1 deficiency alters the gut microbiome response to Western diet |
| title_full | 11β-hydroxysteroid dehydrogenase-1 deficiency alters the gut microbiome response to Western diet |
| title_fullStr | 11β-hydroxysteroid dehydrogenase-1 deficiency alters the gut microbiome response to Western diet |
| title_full_unstemmed | 11β-hydroxysteroid dehydrogenase-1 deficiency alters the gut microbiome response to Western diet |
| title_short | 11β-hydroxysteroid dehydrogenase-1 deficiency alters the gut microbiome response to Western diet |
| title_sort | 11β-hydroxysteroid dehydrogenase-1 deficiency alters the gut microbiome response to western diet |
| topic | Glucocorticoids 11β-HSD Diet Gut microbiome |
| url | https://repository.daystar.ac.ke/handle/123456789/2965 |
| work_keys_str_mv | AT jethrosjohnson 11bhydroxysteroiddehydrogenase1deficiencyaltersthegutmicrobiomeresponsetowesterndiet AT opiyomonican 11bhydroxysteroiddehydrogenase1deficiencyaltersthegutmicrobiomeresponsetowesterndiet AT thomsonmarian 11bhydroxysteroiddehydrogenase1deficiencyaltersthegutmicrobiomeresponsetowesterndiet AT karimgharbi 11bhydroxysteroiddehydrogenase1deficiencyaltersthegutmicrobiomeresponsetowesterndiet AT andreasheger 11bhydroxysteroiddehydrogenase1deficiencyaltersthegutmicrobiomeresponsetowesterndiet AT chapmankarene 11bhydroxysteroiddehydrogenase1deficiencyaltersthegutmicrobiomeresponsetowesterndiet |
Immunology, the microbiome and future perspectives